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KMID : 0811719990030020147
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Korean Journal of Physiology & Pharmacology
1999 Volume.3 No. 2 p.147 ~ p.155
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Antioxidant Effects of Serotonin and L-DOPA on Oxidative Damages of Brain Synaptosomes
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Sang Soo Ham
Dong Hyun Kim/Suk Ha Lee/Yun Sang Kim/Chung Soo Lee
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Abstract
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Antioxidant effects of serotonin and L-DOPA on neuronal tissues were examined by studying the oxidative damages of brain synaptosomal components. The study further explored the mechanism by which they exert protective actions. Serotonin and L-DOPA (1 μM to 1 mM) significantly inhibited lipid peroxidation of brain tissues by either Fe2+ and ascorbate or t-butyl hydroperoxide in a dose dependent fashion. Protective effect of serotonin on the peroxidative actions of both systems was greater than that of L-DOPA. Protein oxidation of synaptosomes caused by Fe2+ and ascorbate was attenuated by serotonin and L-DOPA. Protein oxidation more sensitively responded to L-DOPA rather than serotonin. Serotonin and L-DOPA (100 μM) decreased effectively the oxidation of synaptosomal sulfhydryl groups caused by Fe2+ and ascorbate. The production of hydroxyl radical caused by either Fe3+, EDTA, H_2O_2$ and ascorbate or xanthine and xanthine oxidase was significantly decreased by serotonin and L-DOPA (1 mM). Equal concentrations of serotonin and L-DOPA restored synaptosomal Ca2+ uptake decreased by Fe2+ and ascorbate, which is responsible for SOD and catalase. Protective effects of serotonin and L-DOPA on brain synaptosomes may be attributed to their removing action on reactive oxidants, hydroxyl radicals and probably iron-oxygen complex, without chelating action on iron.
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KEYWORD
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Antioxidant action, Serotonin, L-DOPA, Synaptosomes,
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